ThemaPanel

ThemaPanel Service: Thematic Gene Expression Panels

At Genexpath, we understand that the key to overcoming complex diseases like cancer, cardiovascular disease, and autoimmune disorders lies in a deep understanding of the underlying biological processes. That's why we're proud to announce the upcoming launch of our innovative thematic panels, specifically designed to target crucial mechanisms like inflammation and angiogenesis. These groundbreaking tools, applicable to both humans and mouse models, offer unparalleled precision for your research and clinical applications. Discover how Genexpath can transform your approach to personalized medicine and open new avenues for more effective and targeted treatments.

ThemaPanels will offer you the possibility of studying around 40 genes targeting the biological process concerned using the RT-MLPSeq technique.

What is RT-MLPSeq?

The key points of the protocol

In vitro test 

  • 4 big steps
  • 1/2 day manipulation
  • Sensitive thanks to short probes
  • Adapted to FFPE samples

Sequencing 

  • Adapted to Illumina technology
  • Possible to sequence with others
  • Only 100,000 reads are needed per sample

Analyzed

  • Increased specificity thanks to UMI
  • Bioinformatics analyzes included
  • Access to all raw data

Technical Specifications

Sample types Samples containing mRNA that you wish to quantify
Application domain Gene expression
Handling duration ≃4h (without sequencing)
Actual working time ≃1h-1h30 (without sequencing)
Input quantity Between 50 and 500 ng of RNA in a volume of 2µL
Reagents Probes targeting markers of interest for the theme, barcodes, sequence primer
Material compatibility Sequencer Illumina®
Accessible data A file including the raw data and the relative expression of each marker

Kit Contents

Example of ThemaPanel Kit

What the kit contains:

  • RT-MLPA probe mix
  • Genexpath barcodes
  • Sequencing primers
  • Available in 32 or 64 analyses

Bioinformatics Analyses

  • Access to our RT-MIS platform included

Resources

Gene Expression Profiling In Vitro Test Implementation

Training Video

Contact for More Information

By providing this information, you agree to be contacted so that we can best respond to your requests.

This allows us to provide you with additional personalized information. Your data will be deleted upon simple request.

Contact Us