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RT-MLPSeq: Precision Genetic Analysis
RT-MLPSeq (Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification with High-Throughput Sequencing) is a cutting-edge technology designed for highly precise analysis of genetic markers. This semi-quantitative method enables simultaneous evaluation of multiple biomarkers, including genes, mutations, and chromosomal translocations, using specific oligonucleotide probe pairs. This approach ensures robust and reliable detection, critical for advancing research and diagnosing complex diseases.
Key Steps of RT-MLPSeq
RNA Extraction
Performed under standard laboratory conditions. Compatible with fresh, frozen, or formalin-fixed paraffin-embedded (FFPE) tissue samples.
Reverse Transcription to cDNA
Converts mRNA into complementary DNA (cDNA) for targeted and precise analysis.
Specific Probe Hybridization
Unlike traditional RT-PCR, oligonucleotide probes target exon-exon junctions of cDNA, ensuring specificity without genomic DNA interference. Unique Molecular Identifiers (UMIs) enable accurate quantification of initial molecules.
Ligation and Amplification
Proximal probes are ligated using a ligase and amplified via PCR, incorporating barcodes (BC) for precise sample identification and sequencer-compatible adapters.
Next-Generation Sequencing (NGS)
High-throughput sequencing allows simultaneous analysis of multiple samples and dozens of genes. Requires only 100,000 reads for robust results, analyzed through the RT-MIS bioinformatics platform for rapid, reliable interpretation.
Why Choose RT-MLPSeq?
- Semi-Quantitative Analysis: Provides precise measurement of biomarker expression levels.
- Multi-Marker Profiling: Efficiently detects genes, mutations, and chromosomal translocations in a single assay.
- Enhanced Specificity: Targets exon-exon junctions for accurate detection without genomic interference.
- Versatile Sample Compatibility: Suitable for fresh, frozen, or FFPE tissues, requiring minimal RNA input.
Unlock advanced genetic analysis with RT-MLPSeq for deeper insights into complex genomic profiles.
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