Characterizing cancers
to understand them better

"I'm a clinical hematologist at the Henri Becquerel Center in Rouen, and I'm also involved in translational research at INSERM Unit U1245 in Professor JARDIN's team. I have carried out several research projects on the molecular characterization of lymphomas, using RT-MLPA with the LymphoSign signature to study the gene expression profile of lymphomas. 

At the Henri Becquerel center, the technique LymphoSign test is available in routine use to help the diagnosis of lymphomas and proves to be very valuable for the accurate classification of lymphomas, and also to assist the pathologist in diagnosing difficult cases, which are not so rare in practice. 

I'm particularly interested in primary B lymphomas of the mediastinum (PMBL), which are a unique and rare entity of aggressive mature B-cell lymphoma, preferentially affecting young patients with a female predominance. Diagnosis of these lymphomas is difficult, and the LymphoSign technique is in my view a very useful companion test for accurately identifying and diagnosing these cases, with strong clinical consequences since the treatment and prognosis of these lymphomas is quite different from other aggressive B-cell lymphomas."

Dr Vincent CAMUS , clinical hematologist at the Centre Henri Becquerel in Rouen, France

"La simplicité d’utilisation et la robustesse du kit Lymphosign nous ont permis de l'intégrer efficacement avec notre lymphopanel NGS, facilitant ainsi le travail du pathologiste pour le diagnostic de cas complexes.

Nous avions initialement opté pour la RT-MLPA, qui nous avait convaincus pour l’étude des lymphomes B à grandes cellules. Cependant, le kit Lymphosign a permis d'élargir l'analyse de l'expression aux lymphomes B à petites cellules et aux principales entités des lymphomes T.

De plus, la combinaison de l’analyse d'expression et de l'étude des mutations hotspot permet, dans certains cas, de réduire le recours à l'analyse NGS."

Dr Audrey Gros, MCU-PH, Service de Biologie des tumeurs et Tumorothèque - CHU de Bordeaux; Unité Inserm U1312 - BRIC (BoRdeaux Institute of onCology)- Équipe 5 Trio2 (Recherche translationnelle en cancérologie cutanée et maladies cutanées rares)"

"The study of the gene expression profile of lymphomas using RT-MIS analysis ( lymphosign has been routinely integrated into the laboratory and allows us to finely evaluate the nosological subtype of mature lymphoid hematological diseases. 

This analysis is a particularly useful complement for the anatomo-biological comparisons of complex cases and provides us with information different from high-throughput sequencing analyses. 

The ease of use of the kit from a technical and analytical point of view and its versatility allowed us to easily integrate it into the range of analyzes carried out in our molecular biology laboratory."

Dr Sylvain CARRAS, MD - PhD au CHU Grenoble Alpes