In research projects, it is often necessary to obtain the gene expression of several dozen markers. Currently, relative expression can only be analyzed with a small number of genes or too many genes simultaneously and using complicated protocols. In addition, handling times are often very long and solutions on the market are often expensive.
Genexpath’s CustomPanel service
Genexpath's CustomPanel service allows you to select your markers of interest quickly and easily. We then provide you with a turnkey solution: a test kit in Vitro test and a bioinformatic analysis after a NGS sequencing.
These custom panels can be produced for any type of genome. We are at your disposal to create panels of ten to a hundred markers.
Genexpath puts at your disposal an effective and suitable solution to optimize gene expression analysis in your research projects.
CustomPanel: Accurate measurement of personalized gene expression
The CustomPanel service allows you to precisely measure the expression signature of the genes of interest that you select. Based on ligation-dependent PCR technology (RT-MLPSeq), the test provides researchers with detailed and crucial knowledge of the data collected.
This integrated solution offers you a complete approach, from marker selection to data analysis, facilitating the interpretation of results for your research projects.
CustomPanel Process
Selection of markers
You choose the genes of interest to analyze.
Panel design
Genexpath designs a personalized CustomPanel tailored to the selected markers.
Performing the test
Upon receipt of the kit, you follow the turnkey protocol to perform the analysis in Vitro test.
Data analysis
You upload the FASTQ file from the sequencing to our RT-MIS platform which provides you with a detailed report containing the raw data as well as the relative expression of each of the markers in the panel.
The key points of the protocol
In Vitro test
- 4 big steps
- 1/2 day manipulation
- Sensitive thanks to short probes
- Adapted to FFPE samples
Sequencing
- Adapted to Illumina technology
- Possible to Sequencing with others
- Only 100,000 reads are needed per sample
Analyzed
- Increased specificity thanks to UMI
- Bioinformatics analyzes included
- Access to all raw data
| Type of samples | Samples containing mRNA that you wish to quantify |
| Application domain | Gene expression |
| handling duration | ≃4h (without sequencing) |
| Actual working time | ≃1h-1h30 (without sequencing) |
| Input quantity | Between 50 and 500 ng of ARN in a volume of 2µL |
| Reagents | Probes targeting your markers of interest, barcodes, sequence primer |
| Material compatibility | Sequencer Illumina® |
| Bio informatique analyses | Access to the RT-MIS analysis platform included |
| Accessible data | A file including the raw data and the relative expression of each marker |
What the kit contains:
- Mix of Genexpath probes according to your choices
- Genexpath barcodes
- Sequencing primers
Available in 32 or 64 analyses
Bio informatic analyses
- Access to our RT-MIS platform included
Access to the CustomPanel documents
Why are you asking me those information ?
By providing this information, you agree to be contacted so that we can best respond to your requests. This allows us to provide you with additional personalized information. Your data will be deleted upon simple request.
Publications related to CustomPanel
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Determination of Molecular Subtypes of Diffuse Large B-Cell Lymphoma Using a Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification Classifier: A CALYM Study
PMID: 29054399
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Biological and Clinical Relevance of Associated Genomic Alterations
PMID: 27923841
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Oncogenic events rather than antigen selection pressure
PMID: 27737507
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Next-Generation Sequencing
PMID: 26819451
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Accurate Classification of Germinal Center B-Cell-Like/Activated B-Cell-Like Diffuse Large B-Cell Lymphoma Using a Simple and Rapid Reverse Transcriptase-Multiplex Ligation-Dependent Probe Amplification Assay: A CALYM Study
PMID: 25891505
Training videos
Gene Expression profiling in vitro test implementation